Dissertation Kamylla Caroline Santos

AUTHOR: Kamylla Caroline Santos

TITLE: Association of microneedling with platelet-rich plasma in the treatment of burn scars in rats

ADVISOR: Prof. Dr. Gustavo Henrique Marques Araújo and Profa. Dra. Júlia de Miranda Moraes

DEFENSE DATE: 05/26/2021

ABSTRACT

The scars caused by burns represent a significant public health problem worldwide. These seriously compromise the quality of life because they are closely related to the decline in self-image and functional capacity, triggered by changes in the patient's physical and emotional integrity. The investigation of potential therapies to minimize this situation is of fundamental importance. Microneedling stimulates collagen production through small lesions in the dermis that serve as channels for growth factors present in platelet-rich plasma, effective in accelerating and regenerating tissue. This study aims to evaluate the effects of the association of the microneedling technique, and the application of platelet-rich plasma in the treatment of skin scars caused by third-degree burns. A total of 50 Wistar rats were divided into a control group and groups treated with microneedling, platelet-rich plasma (PRP) and the microneedle association with PRP, after 21 days of a full-thickness burn injury caused by scald in the dorsal region. of the animals. After macroscopic and microscopic analyzes, we concluded that both therapies, alone or associated, were able to significantly influence the process of scar repair in the treated groups. The combination of these therapies can overcome dermal benefits, significantly reducing the treated scar, making it possible to visualize a better organization of the extracellular matrix with a probable collagen deposition by increasing angiogenesis, greater infiltration of mononuclear cells, increased inflammatory signs, in addition to reepithelialization in a more organized.

Keywords: Tissue repair; Burn; Scar, microneedling, platelet-rich plasma