OLIVIA BASSO ROCHA

AUTHOR: OLIVIA BASSO ROCHA

TITLE: CRIOPROTECTORS AND EMBRYOTOXICITY IN ZEBRAFISH (Danio rerio) (CRIOPROTETORES E EMBRIOTOXICIDADE EM ZEBRAFISH (Danio rerio))

ADVISOR: Prof. Dr. Mônica Ferreira Machado

DEFENSE DATE: 10/31/2018

 

ABSTRACT:

Cryopreservation is a technique that develops in dependence on embryonic development and in a process compression, followed by cryoprotective cooling, in order to keep intact the genetic material of the desired cell. Cryopreservation in fish is not yet viable citadella, for several reasons, among them can be cited: embryo size and cryoprotectant cytotoxicity. All tests performed for cryoprotectant toxicity were related to the maintenance of the embryos in different reactions at room temperature. However, there is no data comparing toxicity between temperature increases. Thus, the present work was analyzed on the toxicity of cryoprotectants at room temperature and colds. The testes were separated in zebrafish embryos (Danio rerio) using 12% of the compounds, in serial dilution, of 50% to 0.002%. Observations of deaths and teratogenic changes were made every 24 hours. DMSO and glycerol are the least toxic, but they determine the formation of perimeter edema, when they are used in a greater number of children than 3.12%. At the temperature of cooling, we observed that the toxicity of cryoprotectants decreased and that there was an increase in hatch delay, which was expected, since the low temperatures the embryo development time were reduced. The cryoprotectants were most suitable for cooling were methanol and ethylene glycol, in concentrations of 1.56% to 0.78%.

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