RHAVILLA SANTOS DE OLIVEIRA

AUTHOR: RHAVILLA SANTOS DE OLIVEIRA

TITLE: EVALUATION OF PHOTOBIOMODULATION WITH LASER AsGaAl (670nm) ON FIBROBLAST CULTIVATION EQUINE

ADVISOR:  Prof. Dr. Gustavo Henrique Marques Araujo, Co-Advisor: Prof. Dr. Júlia de Miranda Moraes  

DEFENSE DATE: 10/30/2018

ABSTRACT: The photobiomodulation is a non-invasive therapy, with low cost, using the laser or led light, under the infrared near or red spectrum in low potency densities, in order to promote the activation and modulation of some cellular mechanisms. The laser therapy is commonly used to induce analgesia, reduce inflammatory process and edema, and, to hasten the regeneration of injured tissues as, skin wounds, tendon and bones lesions. The present study aimed to investigate the effects of the photobiomodulation at equine fibroblast primary culture. The fibroblast culture cells were divided in 5 groups (10000 cells/each): control, and irradiated groups with different energy densities 0.5J/cm², 2J/cm², 5J/cm² and 10J/cm². The cells were cultured in a 24 well dish plate, and the groups received one or two laser irradiation 24 hours apart. The samples were obtained after 24h of each irradiation, and before for control. To proceed the cell concentration were used the direct counting with the NeuBauer chamber, trypan blue were applied to access the cellular viability, also was performed the DNA integrity test using the comet assay and, for nuclear alterations were made the Micronucleus test. The viability, concentration and Comet data were analyzed with ANOVA, followed by Tukey test when necessary, and, the chi-square test was used to the micronucleus data, the significance level was 5% in all statistical tests applied. Was concluded that the laser used in the presented optical and energy density parameters had shown some anti-mutagenic effect, and, according the energy density, can interfere at the cellular viability and proliferation. In this way, the laser act as a citoprotector agent, by the reduced DNA damage observed at the laser irradiated equine fibroblast cultured cells.