KAMILA LAUANY LUCAS LIMA

AUTHOR:  KAMILA LAUANY LUCAS LIMA

TITLE: COMPARISON BETWEEN 2 DM2 INDUCTION PROTOCOLS AND EVALUATION OF THE EFFECT OF MODERATE PHYSICAL EXERCISE ON LIPOTOXICITY AND Eicososanoids (COMPARAÇÃO ENTRE 2 PROTOCOLOS PARA INDUÇÃO DO DM2 E AVALIAÇÃO DO EFEITO DO EXERCÍCIO FÍSICO MODERADO SOBRE A LIPOTOXICIDADE E OS EICOSANÓIDES)

ADVISOR: Prof. Dr. Sandra Aparecida Benite Ribeiro, Co-Advisor: Prof. Dr. Denise Silva de Oliveira 

DEFENSE DATE: 10/26/2018

 

ABSTRACT:

The present study aimed to test whether: 1) the high fat diet can induce T2D without the administration of streptozotocin (STZ); 2) the high fat caloric intake causes metabolic, behavioral and morphological changes, similar to t2D and; 3) the practice of moderate physical exercise is effective to reverse the metabolic changes caused by T2D and of the high fat diet. To test these hypotheses, we used 57 Wistar 8 week old rats. They were splited into the five experimental groups: group D (N = 25) who received a single intraperitoneal dose of streptozotocin (STZ diluted in citrate buffer, pH 4.5-25mg / kg body weight of the animal) and were fed with high fat diet. To test the effect only of the high fat diet, the HL group (N = 8) was fed with high fat diet but did not receive the STZ injection. Animals with similar ages were used as control (CO, N = 24) and fed with a balanced diet. At the end of the third week, animals of CO and D and HL groups were subdivided into two other groups: sedentary (SE) and exercised (EF). EF groups were submitted to swimming for 6 weeks. The D-SE, D-EF and HL-SE groups presented significant changes in body weight gain, abdominal fat gain, caloric intake, food efficiency, lipid profile and fasting and capillary glucose levels, when compared to control groups. There was no significant difference in the concentrations of arachidonic acid products and the renal tissue did not present morphological alterations. Physical exercise increased high density lipoproteins (HDL), but did not change others evaluated parameters. We conclude that consumption of the high fat diet when associated with sedentary behavior may cause metabolic alterations similar to T2D and that physical exercise was efficient to improve the lipid profile.

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