GRACIELLE TELES PÁDUA

AUTHOR: GRACIELLE TELES PÁDUA

TITLE: MULTI-RESISTANT ENTEROPATHOGENIC ESCHERICHIA COLI (EPEC) IN CATTLE CARCASSES (ESCHERICHIA COLI ENTEROPATOGÊNICA (EPEC) MULTIRRESISTENTE EM CARCAÇAS DE BOVINOS)

ADVISOR: Prof. Dr. ARIEL EURIDES STELLA

DEFENSE DATE: 08/31/2018

 

ABSTRACT:

Escherichia coli (E. coli) occupies the 2nd place among the microorganisms involved in VAD outbreaks in Brazil and is among the 4 most important worldwide, due to its importance, the goal was to verify the microbiological quality against presence of E. coli in products of animal origin. A total of 365 strains were isolated by dragging swab of 154 carcasses slaughtered in Mineiros - GO municipality. The incidence of E. coli in the collected samples was 81.65%. Of the isolated strains, 16 had the gene (eae), and none had the genes (stx1, stx2). Therefore, their frequency in carcasses was 9.74% (15/154). The 16 strains were tested for the adhesion genes (ToxB, efa1), but none presented these genes. They were also tested for motility where 100% (16/16) were positive, and for hemolysis where an incidence of 37.5% (6/16) was obtained. Regarding the antimicrobial resistance profile, 50 strains were tested, and the antibiotics with the highest percentages of resistance among bacteria were Cephalothin with 82% (41/50), followed by Gentamicin and Amicacin with 26% (13/50) each. None of the samples presented the production of extended spectrum beta-lactamase enzyme. The results demonstrate the presence of multidrug resistant EPEC in carcasses of slaughtered cattle in Mineiros - GO municipality.

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